AP Bio Lab 6 Molecular Biology, need expected results & questions' analyses THANKS!, will give pts to best ans - ap biology lab #10 alternative
Our teacher gave us in the lab yesterday and looked at the plates of bacteria present.
It is expected that questions to be answered at the end of this week, with the exception of Question 9 This laboratory is one of 12 AP Biology Laboratory standard that is used by all classes of AP Bio, so if you are to AP Bio, I want to help, and the results and the statements and answers explain the right questions.
The lab begins:
PANORAMA
In this workshop we will examine some basic principles of molecular biology:
... (Objectives)
INTRODUCTION
The bacterium Escherichia coli (E. coli) is an ideal organism for the molecular geneticist to handle and was used heavily in recombinant DNA research. ...
If the first page is the copy like this, you are entitled.
I know it's a site called www.apbiologylabs.info/, but it costs $ 10. It will give many points for the best and quickest response.
Ap Biology Lab #10 Alternative AP Bio Lab 6 Molecular Biology, Need Expected Results & Questions' Analyses THANKS!, Will Give Pts To Best Ans
1 comments:
Section A
1. + LB, LB, LB / Amp + everyone must have a strong growth in LB / Amp + should have a little less LB/Amp- should not
2. a) + LB LB ensure, through to ensure that the bacteria still viable to be exposed to the plasmid; investigations to protect them from the start to find viable
b) LB/Amp- tests to ensure that no bacteria resistant to ampicillin, before they are exposed to plasmids (for example, who already see their R plasmid DNA), LB / Amp + tests to see if he entered the Plasmid succeeded
c) LB / Amp + growth should be less than BB + have, because although both were exposed to plasmids, not all have the difference between these 2 growth plates should you take an idea how many bacteria, the plasmid
3. Each step indicates the direction, and nearly all the numbers on the procedure for disposal
4. Conditions must exactly for the bacteria, the plasmid, so that environmental factors, that his time in the cold, might affect his time in thewarm when you shake it, if it is too hot or too cold
Section C
1. a) the voltage used will affect the speed with which DNA moves
b will affect) the implementation, how far it moves
c) the amount of DNA that determine how the thickness of the bands
d) reverse polarity to change the direction of DNA that moves
2. I had to ask my teacher for him, and told him to use a longer piece of gel
Ask
1. A plasmid is a fragment of bacterial DNA, which is something that is useful in genetic engineering, scientists can already "reprogram" the bacteria to do the things they normally do not (eg, E. Coli, the insulin produce)
2. Restriction enzymes function of the immune system, such as bacteria, that the pursuit of specific DNA sequences and the recognition sites which cut cut
3. Restriction enzymes from bacteria, the immune function of a bacterium
4. Electricity is supplied by the force with which the DNA is in the gel negative to positive DNA negative, theGel acts like a filter that smaller fragments can move faster so that they more
5. if the DNA starts on the left side is the negative electrode on the left, positive right, 4000 should be closer to the base 400 of the extreme right
6. make the most visible of the DNA, is shown in a light box
7. Using an estimate of a graph of the results, you must use the graph paper in the lab book to page 74 is the logarithmic paper, I suggest that before copying, if possible start.
8. It will be online less, because it is a "single point of DNA is not cut, the fragment is not too far because it is larger
I think no answers, but I have the Article 9, which will not be answered.
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